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Immunological analyses

The Plattform Infection Models offers various immunological analyses. In addition to the analyses listed here, further assays can be offered based on specific research questions and needs.

If you are interested in our services or have any further questions, please contact us.

Services

Cell processing from various organs

For all functional assays, cells (PBMCs - peripheral blood mononuclear cells, MNCs - mononuclear cells - from organs) can be processed and, upon request, preserved from different organs within NHP studies. Processing of PBMCs from other whole blood samples is possible upon agreement. 

Sample Material: Citrate whole blood (PBMCs), lymph nodes, spleen, bone marrow, various intestinal sections, biopsies from the intestine, and upon request.

Prices available upon request.

Immunophenotyping of Various Cells (Flow Cytometry)

Depending on the research question, we design multi-parameter flow panels to characterize cells of the innate and adaptive immune response. This can also be combined with ICS (intracellular cytokine staining).

Measurement Parameters: Available upon request and according to the scientific research topic.


Sample Material: EDTA whole blood; additionally for ICS: citrate whole blood or PBMCs, MNCs from various organs.


Required Volume: 50 microliter (for combinations with ICS, volume depends on the research question and the antigens to be studied).

Prices available upon request.

Quantification of analytes, e.g. antibodies and proteins

We offer the determination of antibodies or proteins using ELISA (Enzyme-linked Immunosorbent Assay) and Western Blot. Whenever possible, we use commercial assays, but there is also the option for in-house establishment.

Sample material and required volume upon agreement.

Prices available upon request.

Quantification of Cytokines, chemokine, etc.

ELISpot Assay (Enzyme linked Immuno Spot Assay)

This highly sensitive method is a widely-used assay for the detection and quantification of e.g. cytokine-producing cells.

Measurement Parameters: IFN-γ

Sample Material: Citrate whole blood, PBMCs, MNCs from various organs.


Required Volume: upon agreement

Prices available upon request.

 

Intracellular Cytokine Staining (ICS, flow cytometry) 

 ICS allows for the examination of antigen-specific T-cell responses using flow cytometry. A major advantage is that, in addition to determining the proportion of cytokine-producing cells, it enables simultaneous analysis of multiple surface markers and various intracellular cytokines/molecules in individual cells.

Measurement Parameters: IFN-γ, TNF-α, IL-2, IL-17, IL-10, IL-4, Granzyme B, Perforin, CD107a (LAMP-1), and upon request.


Sample Material: Citrate whole blood, PBMCs, MNCs from various organs.


Required Volume: upon agreement

Prices available upon request.

 

Bead-based Assays

This method allows for the simultaneous determination of a wide variety of soluble or intracellular proteins, such as cytokines, chemokines, growth factors, etc., using flow cytometry.

Measurement Parameters: Available upon request and according to the scientific research topic

Sample Material: Serum, plasma, or cell culture supernatant.

Required Volume: 25 - 50 microliter.


Prices available upon request.

Functional studies

CFSE proliferation assay

This assay can be used to assess the functionality of different cell populations during disease progression or before and during treatments. Depending on the research topic, the assay can be combined with phenotyping or ICS.

Measurement Parameters: CFSE 

Sample Material: Citrate whole blood or PBMCs, MNCs from various organs.


Required Volume: Depends on the research topic


Prices available upon request.

Contact

Dr. Berit Roshani Postdoctoral Scientist Plattform Infection Models