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CRISPR Injektion in Eizelle Tobias Kahland

Genetic Modification

Genetic Modification

The genetic modification of cells and animals is important for analysing human diseases and mimicking them in animal models. Genetically modified non-human primates allow not only research of diseases but also the preclinical testing of new therapies before they can be applied in humans.

 

Genetic Modification in Common Marmosets

Genetically modified cell and mouse models have revolutionized biomedical research and provided numerous insights into the development of diseases that were otherwise impossible to obtain. However, there are many genes in the human genome that have no genetic or functional equivalent in mice. Diseases affecting these genes can therefore not be studied in the mouse model. Human stem cells are an alternative. However, these cannot develop into an organism only in which many diseases become apparent. Genetically modified non-human primates can close this gap between genetic comparability and the complexity of an entire organism, which is particularly important for therapy development. We use methods of genetic modification such as transgenesis or the "gene scissors" CRISPR/Cas in vitro in stem cells and also apply them in vivo to common marmosets in order to establish a model of genetic deafness in this species in collaboration. This model will be used for the development of novel therapies against deafness.

In Vitro Fertilisation in Common Marmosets

With the help of in vitro fertilisation (IVF), it is possible to create embryos from the eggs and sperm of our common marmosets. These embryos can then be genetically modified using modern technologies such as CRISPR/Cas9. In this way, precise genetic modifications can be carried out, making it possible to create disease models. These genetically modified monkey models are of great importance for research into human diseases, as they can provide insights into disease mechanisms and possible therapeutic approaches. 

Egg Cells and Sperm

Injektion of CRISPR/Cas9 into a Common Marmoset Egg Cell

Analysis of eGFP Transgenic and eGFP Chimeric Common Marmoset Tissue