S3 cell culture laboratory
In the S3 cell culture laboratory, infections with pathogens that can cause serious diseases are studied, including SARS-CoV-2 and Herpes B virus (Macacine alphaherpesvirus-1). Workbenches, ultracentrifuges, and cell culture incubators are available for the production and analysis of virus preparations. Infected cells can be examined using automated microscopy, and a 96-well plate reader is available for detecting the spread of viruses with reporter genes. Besides cell culture work, samples obtained from experimental animals in the S3 animal unit are also prepared and further analyzed in this laboratory.
Selected publications
- Flury P, Krüger N, Sylvester K, Breidenbach J, Al Hamwi G, Qiao J, Chen Y, Rocha C, Serafim MSM, Barbosa da Silva E, Pöhlmann S, Poso A, Kronenberger T, Rox K, O'Donoghue AJ, Yang S, Sträter N, Gütschow M, Laufer SA, Müller CE, Pillaiyar T
Design, Synthesis, and Unprecedented Interactions of Covalent Dipeptide-Based Inhibitors of SARS-CoV‑2 Main Protease and Its Variants Displaying Potent Antiviral Activity.
J Med Chem. 2025 Jan 15. doi: 10.1021/acs.jmedchem.4c02254 - DOI - - Hasan MZ, Claus M, Krüger N, Reusing S, Gall E, Bade-Döding C, Braun A, Watzl C, Uhrberg M, Walter L
SARS-CoV-2 infection induces adaptive NK cell responses by spike protein-mediated induction of HLA-E expression.
Emerg Microbes Infect. 2024 Dec;13(1):2361019 - DOI - - Krüger N, Rocha C, Runft S, Krüger J, Färber I, Armando F, Leitzen E, Brogden G, Gerold G, Pöhlmann S, Hoffmann M, Baumgärtner W
The Upper Respiratory Tract of Felids Is Highly Susceptible to SARS-CoV-2 Infection.
Int J Mol Sci. 2021 Sep 30;22(19):10636 - DOI - - Hoffmann M, Hofmann-Winkler H, Smith JC, Krüger N, Arora P, Sørensen LK, Søgaard OS, Hasselstrøm JB, Winkler M, Hempel T, Raich L, Olsson S, Danov O, Jonigk D, Yamazoe T, Yamatsuta K, Mizuno H, Ludwig S, Noé F, Kjolby M, Braun A, Sheltzer JM, Pöhlmann S
Camostat mesylate inhibits SARS-CoV-2 activation by TMPRSS2-related proteases and its metabolite GBPA exerts antiviral activity.
EBioMedicine. 2021 Mar;65:103255 - DOI -