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Neurohistology

Besides standard paraffin-embedded sections, our neurohistology lab offers cryosectioning of brain and nerve tissue for histological, immunohistochemistry, or immunofluorescence staining, respectively. It is also possible to prepare full frontal sections of comparatively large brains, e.g. from rhesus or cynomolgus monkeys. Depending on the scientific question, various staining methods can be applied, reaching from standard histology, such as hematoxylin & eosin, Nissl-, or myelin-staining, towards immunostaining with particular antibodies in order to detect certain cell-specific (e.g. neurons, astrocytes, microglia, lymphocytes) or other antigens (e.g. viral, GFP-marker protein). These antibodies can either be visualized by enzyme-linked chromogens (e.g. diaminobenzidine) or with fluorescent dyes, also as multiple labeling techniques. They are used, for example, to analyze the influences of viral or vector-based transfections for genetic cell manipulation on the central nervous system on a histomorphological basis. Such genetic modifications are applied i. a. in the development of novel cell and gene therapies or in the field of fundamental brain research, e.g. the regulation of neuronal activity by means of neuro-optogenetics.

The submission form for brain and nerve tissue can be downloaded here:

Submission form

Perfusion-fixed brain block of a rhesus macaque (source: DPZ).
Activated microglia in the brain of a cynomolgus monkey (Macaca fascicularis), immunhistochemistry/DAB for Iba-1 (source: DPZ).
Multichannel-fluorescent staining of a marmoset brain section (Callithrix jacchus) in order to visualize the transgenic expression of an external marker protein (green) within a specific neuronal cell type (red), while double-labeling appears yellow; blue: nuclear DNA (source: DPZ).